Composite

Part:BBa_K4260007:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)


Isoeugenol Monooxygenase, normal coding sequence: promoter, RBS, pelB, Iso and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 101
    Illegal NgoMIV site found at 809
    Illegal NgoMIV site found at 1417
    Illegal AgeI site found at 633
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


Design Notes

No considerations were necessary


Source

The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.


References